MultiPathGAN: Structure Preserving Stain Normalization using Unsupervised Multi-domain Adversarial Network with Perception Loss

Histopathology relies on the analysis of microscopic tissue images to diagnose disease. A crucial part of tissue preparation is staining whereby a dye is used to make the salient tissue components more distinguishable. However, differences in laboratory protocols and scanning devices result in significant confounding appearance variation in the corresponding images. This variation increases both human error and the inter-rater variability, as well as hinders the performance of automatic or semi-automatic methods. In the present paper we introduce an unsupervised adversarial network to translate (and hence normalize) whole slide images across multiple data acquisition domains. Our key contributions are: (i) an adversarial architecture which learns across multiple domains with a single generator-discriminator network using an information flow branch which optimizes for perceptual loss, and (ii) the inclusion of an additional feature extraction network during training which guides the transformation network to keep all the structural features in the tissue image intact. We: (i) demonstrate the effectiveness of the proposed method firstly on H\&E slides of 120 cases of kidney cancer, as well as (ii) show the benefits of the approach on more general problems, such as flexible illumination based natural image enhancement and light source adaptation

Artificial intelligence approach for tomato detection and mass estimation in precision agriculture

Funding: This study was carried out with the support of “Research Program for Agricultural Science & Technology Development” (Project No: PJ013891012020), National Institute of Agricultural Sciences, Rural Development Administration, Republic of Korea.Application of computer vision and robotics in agriculture requires sufficient knowledge and understanding of the physical properties of the object of interest. Yield monitoring is an example where these properties affect the quantified estimation of yield mass. In this study, we propose an image-processing and artificial intelligence-based system using multi-class detection with instance-wise segmentation of fruits in an image that can further estimate dimensions and mass. We analyze a tomato image dataset with mass and dimension values collected using a calibrated vision system and accurate measuring devices. After successful detection and instance-wise segmentation, we extract the real-world dimensions of the fruit. Our characterization results exhibited a significantly high correlation between dimensions and mass, indicating that artificial intelligence algorithms can effectively capture this complex physical relation to estimate the final mass. We also compare different artificial intelligence algorithms to show that the computed mass agrees well with the actual mass. Detection and segmentation results show an average mask intersection over union of 96.05%, mean average precision of 92.28%, detection accuracy of 99.02%, and precision of 99.7%. The mean absolute percentage error for mass estimation was 7.09 for 77 test samples using a bagged ensemble tree regressor. This approach could be applied to other computer vision and robotic applications such as sizing and packaging systems and automated harvesting or to other measuring instruments.Publisher PDFPeer reviewe